Why does my Tetraselmis culture settle to the bottom?

phytonoob

New member
Hi, I am currently setting up a culture of Tetraselmis. My question is what makes cells settle to the bottom?
I have 100mL culture inoculated into 1L f/2 media, kept at 26 deg celsius. The cultures look healthy and green, but after about two days of culture, many seem to be settling at the bottom of the beaker. I doubt the reason for them settling is because they are old cells. Could someone help me?

Thanks :)
 
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