I can't seem to find the article right now, but I remember reading one not long ago that included a study which showed that there was no significant difference in a sandbed of around 3" versus one deeper. Does anyone else recall seeing this, and if so, do you have the link to the article.
Also, I recently set up a new tank and added about 2" of new sand and about an inch of sand that I had removed from an existing tank that I was taking down. The live sand had the detritus stirred out of it before adding it to the new tank. Within 3 days I already had tons of worm tunnels visible in the sand through the front glass. After only a few weeks, it almost looks healthier than the sand bed in my main tank that has been there for almost 2 years now. I can't help but wonder if my old tank would benefit from removing the sand bed and replacing it in this manner ever couple of years. I know there is bound to be quite a bit of detritus build-up underneath rocks and other low flow places. It seems as if the detritus build-up over time may contribute to a decreased porosity of the substrate and more anoxic zones. I've considered just using a gravel vac to clean the detritus from the top 1/2" of the sand bed every few months. I will probably try this before replacing the sand. I did completely gravel vac a 10g tank a few months ago that was plumbed into my main system. This tank had serious cyano problems on the sandbed even though the other tanks seemed to be fine. The flow and lighting were both good, so I could only conclude that there were excess nutrients in the sandbed from detritus. After vacuuming it, a similar occurance happened to that tank as with my new tank. Within just a couple of days, there were tons of new worm tunnels all in the sand and the appearance of the bed did seem much healthier. Also, the cyano quit growing in that tank. This was by no means a controlled experiment and others may have totally different results. The fact that this 10g tank was plumbed into a 250g+ system probably also helped to stabilize it after the vacuuming.