Tetraselmis chui: Ideal Green Phyto for the Reef Aquarium & Sustainable Aquaculture

by | Nov 9, 2022 | Invertebrates | 1 comment

Phytoplankton has infinite applications in the reef aquarium hobby and greater industry. All of the world’s nutrition is largely derived from marine phytoplankton. Marine autotrophs alone are capable of producing the massive amounts of precious fats, proteins, and pigments necessary to support the long-term health and coloration of all other marine life. Live phytoplankton allows for the captive rearing of aphotosynthetic corals, oysters, clams, scallops, porcelain crabs, and other filter feeders. Live phytoplankton enhances the nutritional profile of rotifers, artemia, and copepods so as to allow for the sustainable aquaculture of reef fish and invertebrates. Microalgae can even consume toxins in wastewater, so as to allow its reuse. It is the necessary ambition of the advanced aquarist to seek out the power of phytoplankton. Once harnessed, it can allow for the creation of a great many things…

Phytoplankton’s most profound power is the ability to rapidly assimilate inorganic waste such as ammonia, nitrate, phosphate, and carbon dioxide through the magic of photosynthesis. Microalgal cells are indescribably complex nanofactories capable of creating a seemingly infinite array of proteins, lipids, carbohydrates, pigments, enzymes and other metabolic compounds. Each algae species has its own distinct nutritional profile, which can vary greatly with environmental conditions and specific genetic composition. The aquarist seeking to benefit from phytoplankton will find a lipid-producing species that will also survive in the conditions of the aquarium. As with corn, soybeans, chickens, and pigs, humans have selectively bred various microalgal strains to improve their production capacity and nutritional profile. What is a good nutritional profile? For the reef aquarist and marine aquaculturist alike, fats are king. Polyunsaturated fatty acids (PUFAs), specifically arachidonic acid (ARA), eicosenoic acid (EPA), and docosahexaenoic acid (DHA), are the essential cellular fuel of all marine organisms. Also important are sterols, which allow for the composition of hormones and other nuanced molecules. Ideal microalgal species also produce valuable pigments which act as potent antioxidants, accelerate oxygen absorption, and enhance the coloration of the organisms which consume them. An ideal phytoplankton for the reef aquarium hobby and the aquaculture industry is one that produces these valuable nutritional compounds, but can be cultivated at high densities and remain alive in the fridge.

Microalgae strains are researched, organized, maintained and distributed by government laboratories such as the NOAA Lab in Milford, CT.

Tetraselmis chuii is such an algae.

Tetraselmis chui is a marine chlorophyte. This means that it is a member of ‘Green Algae’ and is  VERY distantly related to contemporary land plants. In reality, Tetraselmis has evolved into its own beautiful uniqueness. Each cell is roughly (12-14um) in size. Tetraselmis chui is biflagellate, meaning it has two cellular ‘whips’ that it can and does use to rapidly whirl around. This species can also anchor itself to a substrate (often in response to low flow or high light), forming a biofilm which in and of itself has unique properties/behaviors. As a whole, Tetraselmis chuii has a relatively elastic range of both temperature and salinity. Overall it should be noted that the species survives and thrives at reef aquarium salinity (1.0263) and temperature (78-80F). In fact, Milione et al 2008 demonstrated that such water quality conditions coupled with high light intensity was associated with elevated lipid production in Tetraselmis chui. Furthermore, it has been demonstrated that nitrogen/phosphorus starvation stimulates cellular storage of PUFAs. This suggests that when live Tetraselmis cells are dosed into an oligotrophic reef tank…they immediately begin to swell with fatty goodness…becoming perfect packaged pillows of cellular nutrition.

Tetraselmis chui in Global Aquaculture

Tetraselmis chui has classically been used as a live feed for the larviculture of commercial shellfish species such as the eastern oyster, (Crassostrea virginica), hardshell clam (Mercenaria mercenaria), eastern bay scallop (Argopectin irradians) and European flat oyster (Ostrea edulis). It has also been used to rear the larvae of various tropical mollusc species such as giant clams (Tridacna sp.), gold-lipped pearl oyster (Pinctada maxima), Sydney rock oyster (Saccostrea commercialis) and the milk conch (Strombus costatus), as well as several marine tube worms in the Serpulidae and Spirorbidae. Abdelaty et al. 2021 used live Tetraselmis cells to rear the larvae of the tropical sea cucumber Holothuria scabra. Tetraselmis is especially helpful with these species when fed in the weeks/days prior to larval settlement as it provides sterols such as  24-methylenecholesterol and 24-methylcholesterol. These are made to construct hormones. It is becoming growingly known that such specific dietary cues derived from microalgae are crucial for successful metamorphosis.

“…high-lipid Tetraselmis strains supported significantly faster oyster growth than an equivalent ration of T-ISO. Doubling times for oyster weight, volume, and shell height were in the range of 1.5–3 weeks on single daily feedings of the best diets. Tetraselmis strains yielding the most rapid oyster growth contained higher contents of the essential fatty acid 20:5n − 3 and of the sterols 24-methylcholesterol and/or 24-methylenecholesterol. These data are consistent with our earlier findings that these compounds appear to be deficient in most phytoplankton for optimal growth of Crassostrea virginica spat.” (Wikfors et al 1996)

Tetraselmis chui has been widely deployed in the realm of shrimp farming feeding the larvae of species like the pacific white-legged shrimp (Litopenaeus vannamei), Atlantic whie shrimp (Penaeus setiferus) and Atlantic brown shrimp (Farfantepenaeus aztecus). Adding live Tetraselmis cells enhances the performance of shrimp production ponds through stabilizing water quality and reducing pathogenic bacterial loads. This concept carries on into the world of ornamental shrimp production, particularly in the aquaculture of ‘frontier’ reef species. Simoes et al. 2002 used Tetraselmis chui to enrich the rotifers fed to the larvae of fire shrimp (Lysmata debelius). Cunha et al 2000 proposed a similar approach for rearing cleaner shrimp (Lysmata amboinensis) larvae.

Tetraselmis’ most profound service to aquaculture allowed for breeding of seemingly impossible reef fish species to cultivate. It does this by enhancing the nutritional profile of zooplankton live feeds such as rotifers (Brachionus sp.), brine shrimp (Artemia sp.), ciliates (Fabrea salina), sea fleas (Moina sp.), harpacticoid copepods (Tisbe sp. Tigriopus sp.), cyclopoid copepods (Apocyclops sp.) and calanoid copepods (Acartia sp., Parvocalanus sp., Pseudodiaptomus sp.). If these zooplankton are fed to marine fish larvae while surrounded by live Tetraselmis cells, then they are constantly gut-loaded with lipids, sterols and other algae goodness. Living algae cells are also photosynthesizing algae cells–meaning they are actively absorbing ammonia, nitrates, phosphates and carbon dioxide.  Moreso, the living algae cells provide shade from light and reduce occurrences of larval gression/cannibalism. Using live microalgae cells for this nutritional/chemical/ behavioral modulation is a technique referred to as Greenwater Larviculture. And because of the immaculate abilities of chlorophytes, such as Tetraselmis chui, hundreds of marine species can now be reared completely in captivity. This practice has led to the successful aquaculture of fish species such as the mediterranean sea bream (Sparus aurata),  melanurus wrasse (Halichoeres melanurus) and leopard grouper (Plectropomus leopardus) to name but a few. Perhaps most notably was the contribution of Tetraselmis chui as a live feed in the aquaculture of the pacific blue tang (Paracanthrus hepatus) by Dimaggio et al in 2017 at the University of Florida. Such frontier cultivation efforts have ushered in a new age of sustainable aquaculture and sustainable reef keeping.

Tetraselmis chui in the Reef Aquarium


Live Tetraselmis chui cells are a truly ‘functional’ food in the reef aquarium. Not only do they enhance nutrition to any copepods, rotifers or artemia fed to the aquarium, but microalgae cells can and will be directly consumed by a wide variety of corals and invertebrates. In suspension, microalgae cells can be grazed on sponges, porcelain crabs, sabellid worms, clams and corals. What few cells that may form a biofilm act as an incredible nutrition source for clean up crew snails, conchs and urchins. Just like in greenwater larviculture, every second a living Tetraselmis cell goes uneaten, it eats. Only it feasted upon dissolved phosphates, nitrates, ammonia, carbon dioxide. The general environmental conditions of a reef aquarium (high light, high salinity, low nutrients) encourage living cells to grow and develop large reserves of golden PUFA fats.

It is well documented but rarely celebrated that microalgae can grow heterotrophically and act to directly consume waste organic carbon. This combined with its aggressive photosynthetic potential, makes Tetraselmis a great wastewater processing species. Many successful trials have demonstrated the ability of both suspended and stationary Tetraselmis cells to strip nutrients and even heavy metals from aquacultural/agricultural, municipal and industrial wastewater. In the aquarium, this thought process can be translated into the recycling of water change wastewater. Rather than releasing it into local sewer systems or dumping it outside, this expensive waste can be sterilized and added to a phytoreactor–reclaiming at least some of the lost resource. El-Sayid et al 2020 identified Tetraselmis chui as a ‘promising marine algal candidate for the dual use of petroleum wastewater treatment and biodiesel production’. Who knows…perhaps the reef aquariums of the future will be powered by algae-generated electricity!

In addition to modulating water quality as it radiates nutrition, there is growing evidence that Tetraselmis, and other microalgae, produce antimicrobial compounds which suppress the growth of pathogenic bacteria strains. Pathogenic bacteria, such as Vibrio sp., are a constant daunting challenge to aquarium keepers as they can induce catastrophic disease to fish, corals and invertebrates alike. Kokou et al 2012 demonstrated that extract from Tetraselmis chui cells inhibited the growth of Vibrio parahaemolyticus, V. anguillarum, V. splendidus, V. alginolyticus, V. scopthalmi and V. lentus in petri dish experiments. Malign et al 2011 attributed the following fattu acids: pentadecanoic acid (methyl pentadecanoate), 1,13-tetradecadiene, 9-hexadecenoic acid, methyl palmitoleate, 9- Hexadecenoic acid, methyl palmitoleate hexadecanoic acid; palmitic acid9,12- octadecadienoic acid (linoleic acid), 9-octadecenoic acid (oleic acid), 1,2- benzenedicarboxylic acid with suppression of Echseri coli and Staphylococcus aureus. Widowati et al 2018 observed reductions of Vibrio alginolyticus infection in Litopenaeus vannamei fed Tetraselmis chui and Dunaellia salina cells for 21 days. Farisa et al 2019 demonstrated reduced Vibrio harveyi levels in rotifers (Brachionus plicatilis) fed live Tetraselmis diets. Makradis et al 2006 observed reduced Vibrio loads in Artemia cultures fed live Tetraselmis cells. This suggests a cleaning affect a live microalgae diet can have in conjunction to its nutritional contribution to zooplankton live feeds. Cerezuela et al 2012 suggested that Tetraselmis had the potential to provide immunostimulation to fish and this has been well observed in commercial shrimp farming. A question that remains to be thoroughly scientifically evaluated is:

Can Tetraselmis play a role in resolving coral disease?


Tetraselmis chui is a Green Phyto of unbelievable promise to both the reef aquarium industry as well as global commercial aquaculture. Few of the millions of algae species have that sweet combination of rapid growth, elastic environmental tolerances and lipid-rich nutritional profile. This microalgae species will allow for the cultivation of many more species…not only reef fish which desperately need to be aquacultured, but all sorts of filter feeding organisms previously unavailable to the hobby altogether. Grown on a small scale, it can be used to deliver a bioactive feeding to even the smallest-mouthed reef inhabitant. On a large scale, it has the potential to cleanse wastewater and push the hobby…and industry into a Greener Age.

Literature Consulted

Abdelaty, M., Al-Solami, L., Al-Harbi, M., & Abu El-Regal, M. (2021). Utilization of Different Types of Microalgae to Improve Hatcheries Production of the Sea Cucumber Holothuria scabra Jaeger, 1833 in the Red Sea, Egypt. Egyptian Journal of Aquatic Biology and Fisheries, 25(2), 193-204.

Aranda, D. A., Lucas, A., Brule, T., Salguero, E., & Rendon, F. (1989). Effects of temperature, algal food, feeding rate and density on the larval growth of the milk conch (Strombus costatus) in Mexico. Aquaculture, 76(3-4), 361-371.

Benvenuti, G., Bosma, R., Cuaresma, M., Janssen, M., Barbosa, M. J., & Wijffels, R. H. (2015). Selecting microalgae with high lipid productivity and photosynthetic activity under nitrogen starvation. Journal of Applied Phycology, 27(4), 1425-1431.

Burgess, A. I., Callan, C. K., Touse, R., & Delos Santos, M. (2020). Increasing survival and growth in larval leopard coral grouper (Plectropomus leopardus) using intensively cultured Parvocalanus crassirostris nauplii. Journal of the World Aquaculture Society, 51(1), 171-182.

Cerezuela, R., Guardiola, F. A., González, P., Meseguer, J., & Esteban, M. Á. (2012). Effects of dietary Bacillus subtilis, Tetraselmis chuii, and Phaeodactylum tricornutum, singularly or in combination, on the immune response and disease resistance of sea bream (Sparus aurata L.). Fish & Shellfish Immunology, 33(2), 342-349.

Cunha, L., Mascaro, M., Chiapa, X., Costa, A., & Simoes, N. (2008). Experimental studies on the effect of food in early larvae of the cleaner shrimp Lysmata amboinensis (De Mann, 1888)(Decapoda: Caridea: Hippolytidae). Aquaculture, 277(1-2), 117-123.

DiMaggio, M. A., Cassiano, E. J., Barden, K. P., Ramee, S. W., Ohs, C. L., & Watson, C. A. (2017). First record of captive larval culture and metamorphosis of the Pacific blue tang, Paracanthurus hepatus. Journal of the World Aquaculture Society, 48(3), 393-401.


Farisa, M. Y., Namaskara, K. E., & Yusuf, M. B. (2019, March). Antibacterial potention of extract of rotifers fed with different microalgae to control Vibrio harveyi. In IOP Conference Series: Earth and Environmental Science (Vol. 246, No. 1, p. 012058). IOP Publishing.

Gallardo, P. P., Alfonso, E., Gaxiola, G., Soto, L. A., & Rosas, C. (1995). Feeding schedule for Penaeus setiferus larvae based on diatoms (Chaetoceros ceratosporum), flagellates (Tetraselmis chuii) and Artemia nauplii. Aquaculture, 131(3-4), 239-252.

Hamer, J. P. (2002). The development and settlement of certain marine tubeworm (Serpulidae and Spirorbidae) larvae in response to biofilms. Bangor University (United Kingdom).

Khairy, H. M., & El-Sayed, H. S. (2012). Effect of enriched Brachionus plicatilis and Artemia salina nauplii by microalga Tetraselmis chuii (Bütcher) grown on four different culture media on the growth and survival of Sparus aurata larvae. African Journal of Biotechnology, 11(2), 399-415.

Khatoon, H., Haris, H., Rahman, N. A., Zakaria, M. N., Begum, H., & Mian, S. (2018). Growth, proximate composition and pigment production of Tetraselmis chuii cultured with aquaculture wastewater. Journal of Ocean University of China, 17(3), 641-646.

Kokou, F., Makridis, P., Kentouri, M., & Divanach, P. (2012). Antibacterial activity in microalgae cultures. Aquaculture Research, 43(10), 1520-1527.

Kuban, F. D., Lawrence, A. L., & Wilkenfeld, J. S. (1985). Survival, metamorphosis and growth of larvae from four penaeid species fed six food combinations. Aquaculture, 47(2-3), 151-162.

Lu, L., Wang, J., Yang, G., Zhu, B., & Pan, K. (2017). Heterotrophic growth and nutrient productivities of Tetraselmis chuii using glucose as a carbon source under different C/N ratios. Journal of Applied Phycology, 29(1), 15-21.

Maligan, J. M., Saksony, A. K., & Widayanti, V. T. (2011). Identification Of Antioxidant and Antibacteria Activity of Marine Microalgae Tetraselmis chuii Extract. In 5th Young Scientist Seminar (pp. 25-6).

Makridis, P., Costa, R. A., & Dinis, M. T. (2006). Microbial conditions and antimicrobial activity in cultures of two microalgae species, Tetraselmis chuii and Chlorella minutissima, and effect on bacterial load of enriched Artemia metanauplii. Aquaculture, 255(1-4), 76-81.

Marques, D., Albuquerque, R., Correia, C., Barraca, C., Aguilar, F., & Leandro, S. (2014). Effect of different diets and rearing tanks on the development and larval survival of Lysmata amboinensis (De Mann, 1888). In Front. Mar. Sci. Conference Abstract: IMMR| International Meeting on Marine Research.

Masuda, K., Miyachi, S., & Maruyama, T. (1994). Sensitivity of zooxanthellae and non-symbiotic microalgae to stimulation of photosynthate excretion by giant clam tissue homogenate. Marine Biology, 118(4), 687-693.

Matias da Cunha Costa, J. R., Ratcliff, S., Tsuzuki, M. Y., & Schwarz, M. H. (2021). Co-feeding schemes affect survival, growth, and development in the larviculture of the Striped cleaner shrimp Lysmata amboinensis (de Mann, 1888). Journal of Applied Aquaculture, 1-14.

Meseck, S. L., Wikfors, G. H., Alix, J. H., Smith, B. C., & Dixon, M. S. (2007). Impacts of a cyanobacterium contaminating large-scale aquaculture feed cultures of Tetraselmis chui on survival and growth of bay scallops, Argopecten irradians irradians. Journal of Shellfish Research, 26(4), 1071-1074.

Milione, M., Zeng, C., & Tropical Crustacean Aquaculture Research Group. (2007). The effects of algal diets on population growth and egg hatching success of the tropical calanoid copepod, Acartia sinjiensis. Aquaculture, 273(4), 656-664.

Milione, M., & Zeng, C. (2008). The effects of temperature and salinity on population growth and egg hatching success of the tropical calanoid copepod, Acartia sinjiensis. Aquaculture, 275(1-4), 116-123.

Mohammadi, M., Kazeroni, N., & Baboli, M. J. (2015). Fatty acid composition of the marine micro alga Tetraselmis chuii Butcher in response to culture conditions. J. Algal. Biomass Utln, 6, 49-55.

O’Connor, W. A., Nell, J. A., & Diemar, J. A. (1992). The evaluation of twelve algal species as food for juvenile Sydney rock oysters Saccostrea commercialis (Iredale & Roughley). Aquaculture, 108(3-4), 277-283.

Patterson, G. W., Tsitsa-Tzardis, E., Wikfors, G. H., Gladu, P. K., Chitwood, D. J., & Harrison, D. (1993). Sterols of Tetraselmis (Prasinophyceae).

Rose, R. A., & Baker, S. B. (1994). Larval and spat culture of the Western Australian silver-or goldlip pearl oyster, Pinctada maxima Jameson (Mollusca: Pteriidae). Aquaculture, 126(1-2), 35-50.

Simoes, F., Ribeiro, F., & Jones, D. A. (2002). Feeding early larval stages of fire shrimp Lysmata debelius (Caridea, Hippolytidae). Aquaculture International, 10(5), 349-360.

Suchar, V. A., & Chigbu, P. (2006). The effects of algae species and densities on the population growth of the marine rotifer, Colurella dicentra. Journal of experimental marine biology and ecology, 337(1), 96-102.

Widowati, I., Zainuri, M., Kusumaningrum, H. P., Maesaroh, Y., Hardivillier, Y., Leignel, V., … & Mouget, J. L. (2018). Identification of agents causing vibriosis in Litopenaeus vannamei shrimp culture in Kendal, Central Java, Indonesia and application of microalgae Dunaliella salina and Tetraselmis chui as bio-control agents against vibriosis. Aquaculture, Aquarium, Conservation & Legislation, 11(1), 101-107.

Widowati, I., Zainuri, M., Kusumaningrum, H. P., Hardivillier, Y., Leignel, V., Bourgougnon, N., & Mouget, J. L. (2021). Growth of shrimp infected by Vibrio, fed with formulated feed with inclusions of Dunaliella salina and Tetraselmis chuii extracts. Aquaculture, Aquarium, Conservation & Legislation, 14(2), 981-987.

1 Comment

  1. David Taliaferro

    Could you please site a source for your statement “microalgae cells can and will be directly consumed by a wide variety of corals”?

    Many people say this, and many people insist that no corals directly consume microalgae (phytoplankton), saying that there has never been any evidence that this is true. If you know of such evidence, I would love to hear about it!

    Thank you.

    – Dave


Submit a Comment

Your email address will not be published. Required fields are marked *

Upcoming Events