Don't know if this helps but Chris posted this on post #56
Nate, are you shaking it up too much? It's nothing more than a gentle swirl for a couple of seconds. The differing readings are probably air bubbles or differences in time the regent is in the water.
Are you cleaning out the culvet right away after the test? The regeant stains the bottom of the tube. Rinse it right away in FW then clean the inside out with a Q-Tip. I was getting strange readings at first, then I noticed the blue tint on the bottom.
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